Directed Evolution of Methanomethylophilus alvus Pyrrolysyl-tRNA Synthetase Generates a Hyperactive and Highly Selective Variant

J.T. Fischer, D. Söll, J.M. Tharp

Front. Mol. Biosci. 2022, 9, 850613.

[Article link]

Initiating Protein Synthesis with Noncanonical Monomers In Vitro and In Vivo

J.M. Tharp,* J.A. Walker,* D. Söll, A. Schepartz

Method. Enzymol. 2021, 656, 495–519.

[Article link]

Genetic Encoding of Three Distinct Noncanonical Amino Acids Using Reprogrammed Initiator and Nonsense Codons

J.M. Tharp, O. Vargas-Rodriguez, A. Schepartz, D. Söll

ACS Chem. Biol. 2021, 16, 766–774.

[Article link]

An Amber Obligate Active Site-Directed Ligand Evolution Technique for Phage Display

J.M. Tharp,* J.T. Hampton,* C.A. Reed,* A. Ehnbom, P.-H.C. Chen. J.S. Morse, Y. Kurra, L.M. Pérez, S. Xu, W.R. Liu

Nature Commun. 2020, 11, 1392.

[Article link]

Hijacking Translation Initiation for Synthetic Biology

J.M. Tharp,* N. Krahn,* U. Varshney, D. Söll

ChemBioChem, 2020, 21, 1287–1396.

Selected as a Very Important Paper (VIP)

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Initiation of Protein Synthesis with Non-Canonical Amino Acids In Vivo

J.M. Tharp, O. Ad, K. Amikura, F.R. Ward, E. Garcia, J.H.D. Cate, A. Schepartz, D. Söll

Angew. Chem. Int. Ed. 2020, 59, 3122-3126.

[Article link]

Engineering Aminoacyl-tRNA Synthetases for Use in Synthetic Biology

N. Krahn,* J.M. Tharp,* A. Crnković,* D. Söll

The Enzymes, 2020, 48, 351–359.

[Article link]

A Genetically Encoded Phage-Displayed Cyclic-Peptide Library

X.S. Wang, P.S.C. Chen, J.T. Hampton, J.M. Tharp, C.A. Reed, S.K. Das, D.S. Wang, H.S. Hayatshahi, Y. Shen, J. Liu, W.R. Liu 

Angew. Chem. Int. Ed. 2019, 131, 16061-16056.

[Article link]

Using Amber and Ochre Nonsense Codons to Code Two Different Noncanonical Amino Acids in One Protein Gene

J.M. Tharp, W.R. Liu 

Methods Mol. Biol. 2018, 1728, 147-154.

[Article link]

tRNAPyl: Structure, Function, and Applications

J.M. Tharp, A. Ehnbom, W.R. Liu 

RNA Biol. 2018, 15, 441-452.

[Article link]

Genetic Code Expansion: Synthetases Pick Up the PACE

J.M. Tharp, W.R. Liu 

Nat. Chem. Biol. 2017, 13, 1205-1206.

[Article link]

A Genetically Encoded Allysine for the Synthesis of Proteins with Site-Specific Lysine Dimethylation

Z.A. Wang, Y. Kurra, X. Wang, J.M. Tharp, E.C. Vatansever, W.W. Hsu, S. Dai, X. Fang, W.R. Liu 

Angew. Chem. Int. Ed. 2016, 56, 212-216.

[Article link]

Genetically Encoded Fluorophenylalanines Enable Insights Into the Recognition of Lysine Trimethylation by an Epigenetic Reader

Y.J. Lee, M.J. Schmidt, J.M. Tharp, A. Weber, H. Zheng, J. Gao, M.L. Waters, D. Summerer, W.R. Liu 

Chem. Commun. 2016, 52, 12606-12609.

[Article link]

The "π-Clamp" Offers a New Strategy for Site-Selective Protein Modification

J.M. Tharp, W.R. Liu 

ChemBioChem 2016, 17, 883-885.

[Article link]

Expanding the Chemical Diversity of Lasso Peptide MccJ25 with Genetically Encoded Non-Canonical Amino Acids

F.J. Piscotta, J.M. Tharp, W.R. Liu, J.A. Link 

Chem. Commun. 2015, 51, 409-412.

[Article link]

Genetic Incorporation of Seven Ortho-Substituted Phenylalanine Derivatives

J.M. Tharp, Y.S. Wang, Y.J. Lee, Y.Y. Yang, W.R. Liu 

ACS Chem. Biol. 2014, 9, 884-890.

[Article link]

Pyrrolysyl-tRNA Synthetase: An Ordinary Enzyme but an Outstanding Genetic Code Expansion Tool

W. Wan, J.M. Tharp, W.R. Liu 

Biochim. Biophys. Acta 2014, 1844, 1059-1070.

[Article link]

Developmental Plasticity of Thermal Tolerances in Temperate and Subtropical Populations of Drosophila melanogaster

B.S. Cooper, J.M. Tharp, I.I. Jernberg, M.J. Angilletta 

J. Therm. Biol. 2012, 37, 211-216.

[Article link]

* signifies equal author contribution